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FOR2143


Institute of Physiology
University of Freiburg
Hermann-Herder-Str. 7
79104 Freiburg
Germany
How to find us

 

Physiology I
Prof. Dr. Marlene Bartos
Tel.: +49 (0)761 203 5194
Fax.: +49 (0)761 203 5204

 

Physiology II
Prof. Dr. Bernd Fakler
Tel. +49 761 203-5176
Fax +49 761 203-5191
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Techniques

Research techniques used by our group

Electrophysiology

We apply various configurations of the patch-clamp technique (whole-cell, cell-attached, excised inside-out, outside-out) to native and genetically manipulated cells and subcellular compartments. They enable us to monitor protein function and protein-protein interactions at high-resolution.

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Membrane Proteomics

We use a large spectrum of biochemical techniques to detect and quantify membrane proteins (mainly ion channels and receptors), their post-translational modifications and association with other proteins in complexes and protein networks.

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Mass Spectrometry

Modern mass spectrometers coupled with liquid chromatography enable us to identify several hundreds of proteins from complex samples with high confidence and sequence coverage. In addition, they provide quantitative data that let us determine stability, specificity and stoichiometry of protein-protein interactions as well as absolute protein abundance.

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NMR Spectroscopy

Nuclear magnetic resonance spectroscopy (NMR) provides information on structure and dynamics of biological macromolecules at atomic resolution under near-physiological conditions. We use it to examine proteins participating in the nano-environment of membrane proteins with regard to their 3D structure, mobility and interactions.

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