Logo SFB 746

Institute of Physiology
University of Freiburg
Hermann-Herder-Str. 7
79104 Freiburg
Germany
How to find us

Physiology I
N.N.

Physiology II
Prof. Bernd Fakler
Tel. +49 761 203-5176
Fax +49 761 203-5191
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  • We apply various configurations of the patch-clamp technique (whole-cell, cell-attached, excised inside-out, outside-out) to native and genetically manipulated cells and subcellular compartments. They enable us to monitor protein function and protein-protein interactions at high-resolution.
  • We use a large spectrum of biochemical techniques to detect and quantify membrane proteins (mainly ion channels and receptors), their post-translational modifications and association with other proteins in complexes and protein networks.
  • Modern mass spectrometers coupled with liquid chromatography enable us to identify several hundreds of proteins from complex samples with high confidence and sequence coverage. In addition, they provide quantitative data that let us determine stability, specificity and stoichiometry of protein-protein interactions as well as absolute protein abundance.
  • Nuclear magnetic resonance spectroscopy (NMR) provides information on structure and dynamics of biological macromolecules at atomic resolution under near-physiological conditions. We use it to examine proteins participating in the nano-environment of membrane proteins with regard to their 3D structure, mobility and interactions.
  • Using innovative microsystems, we work to enhance resolution and throughput of electrical recording from biomembranes. We develop biohybrid sensing devices based on arrays of single biological nanopores in membrane microarrays and study the interaction of semiconductor nanoparticles (quantum dots) with synthetic and biological membranes.
  • To understand how neurons collectively process information, we develop optogenetic tools as well as new technologies for recordings from neurons in vivo and imaging of cell activity using photon Ca2+ and functional approaches. With computational network models we gain information on the principles underlying information processing in complex neuronal circuits.

Chairs

Physiology I

N.N.

Physiology II – Molecular Physiology

Prof. Dr. Bernd Fakler

Our central goal is comprehensive understanding of organization and operation of rapid signal transduction and information processing at the plasma membrane of excitable cells under normal and pathophysiological conditions.

Associated Research Groups

Membrane Physiology and -Technology

Prof. Dr. Jan Behrends

Using innovative microsystems we work to enhance the resolution and throughput of electrical recording from biomembranes.

Information Processing in Neural Networks

Prof. Dr. Marlene Bartos

How is information processed and encoded in neuronal networks to realize learning, memory and behaviour? We aim to uncover the mechanisms underlying information processing by applying electrophysiological, imaging, molecular and computational approaches.

Renal Hemodynamics

PD Dr. Armin Just

We study the mechanisms governing blood flow in the kidney. Our main interest pertains to the mediators and modulators of renal blood flow autoregulation.

Recent Activities
Regional Diversity and Developmental Dynamics of the AMPA-Receptor Proteome in the Mammalian Brain Regional Diversity and Developmental Dynamics of the AMPA-Receptor Proteome in the Mammalian Brain Sep 19, 2014
Jochen Schwenk, et al. Bernd Fakler, Uwe Schulte Neuron (2014), http://dx.doi.org/10.1016/j.neuron.2014.08.044
Cornichon2 Dictates the Time Course of Excitatory Transmission at Individual Hippocampal Synapses Cornichon2 Dictates the Time Course of Excitatory Transmission at Individual Hippocampal Synapses May 22, 2014
Sami Boudkkazi, Aline Brechet, Jochen Schwenk,Bernd Fakler, Neuron 82(4), p848-858; http://dx.doi.org/10.1016/j.neuron.2014.03.031
More…
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