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You are here: Home News Mitochondrial complexome reveals quality-control pathways of protein import
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Mitochondrial complexome reveals quality-control pathways of protein import

Schulte et al., Nature (2023)

Feb 01, 2023

Mitochondrial complexome reveals quality-control pathways of protein import

Schulte et al - Nature 2023

Mitochondria have crucial roles in cellular energetics, metabolism, signalling and quality control. They contain around 1,000 different proteins that often assemble into complexes and supercomplexes such as respiratory complexes and preprotein translocases.

The composition of the mitochondrial proteome has been characterized; however, the organization of mitochondrial proteins into stable and dynamic assemblies is poorly understood for major parts of the proteome. Here we report quantitative mapping of mitochondrial protein assemblies using high-resolution complexome profiling of more than 90% of the yeast mitochondrial proteome, termed MitCOM.

An analysis of the MitCOM dataset resolves >5,200 protein peaks with an average of six peaks per protein and demonstrates a notable complexity of mitochondrial protein assemblies with distinct appearance for respiration, metabolism, biogenesis, dynamics, regulation and redox processes. We detect interactors of the mitochondrial receptor for cytosolic ribosomes, of prohibitin scaffolds and of respiratory complexes. The identification of quality-control factors operating at the mitochondrial protein entry gate reveals pathways for preprotein ubiquitylation, deubiquitylation and degradation. Interactions between the peptidyl-tRNA hydrolase Pth2 and the entry gate led to the elucidation of a constitutive pathway for the removal of preproteins.

The MitCOM dataset is a comprehensive resource for the identification, organization and interaction of mitochondrial machineries and pathways.

Nature Research Briefing (Article Summary)

https://doi.org/10.1038/d41586-023-00095-0

Main Article

https://doi.org/10.1038/s41586-022-05641-w

Explore MitCOM Dataset

https://www.complexomics.org/datasets/mitcom

 

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